PCR Supplies
Sigma Aldrich Fine Chemicals Biosciences DNA Polymerase I, Klenow Fragment from Escherichia coli buffered aqueous glycerol solution | 9012-90-2 | MFCD00283091 | 50UN
DNA Polymerase I, Klenow Fragment from Escherichia coli buffered aqueous glycerol solution | 9012-90-2 | MFCD00283091 | 50UN
Agilent Technologies 10X TAQ DNA POLYMERASE BUFFER
10x Taq DNA Polymerase Buffer, Standard buffer containing magnesium, Ideal for optimal PCR performance, Optimized for use with specific PCR enzymes.
Agilent Technologies PFUTURBO DNA POLYMERASEPFUTUR
PfuTurbo DNA Polymerase, 100U (40 rxn), Contents: PfuTurbo DNA polymerase 10X cloned Pfu buffer, Applications: High performance PCR, For high fidelity PCR and primer-extension reactions, PCR cloning and generating blunt-end amplification pr
Agilent Technologies PFUTURBO HOTSTART DNA POLY
PfuTurbo Hotstart DNA Polymerase 1 000 U Hot-start version of PfuTurbo DNA polymerase for added specificity Contains: PfuTurbo Hotstart DNA polymerase 10X cloned Pfu buffer Applications High-fidelity PCR cloning High-fidelity PCR
Cayman Chemical N-(p-Tosyl)-GPR-pNA (acetate), Purity: ≥98%, CAS: 86890-95-1, 25 mg
A colorimetric thrombin substrate; cleavage of the GPR peptide sequence releases pNA, which can be quantified by colorimetric detection at 405 nm as a measure of thrombin activity
As One International Inc 25 mM MgCl2, 10 x 1.5 ml
25mM magnesium is for optimization of magnesium concentrations in PCR reactions. Optimal magnesium concentration increases fidelity and specificity of the polymerase. Too low of a concentration inactivates the polymerase while too high of a concentration increase nonspecific bands. Several variables effect the optimal concentration of magnesium, DNA quality, presence of chelators, and dNTP concentration.
Agilent Technologies PFUULTRA HIGH-FI DNA POLYMPFU
PfuUltra high-fidelity DNA polymerase is a formulation containing a mutant of Pfu DNA polymerase that exhibits enhanced proofreading capability. Ideal for PCR cloning, site-directed mutagenesis and other applications requiring high accuracy
Roche Diagnostics KAPA HiFi HotStart ReadyMix PCR Kit (1.25 mL), 50 x 50 uL reactions
KAPA HiFi HotStart ReadyMix PCR Kit (1.25 mL), 50 x 50 uL reactions. *This item is non returnable.
Qiagen Beverly Inc T4 DNA POLYMERASE
DNA Polymerase; T4 DNA Polymerase; Conc. 3,000 U/ml; 2,000 U; incl. 10X buffer
Sigma Aldrich Fine Chemicals Biosciences T7 RNA Polymerase from Esc
T7 RNA polymerase is commonly used to transcribe DNA which has been cloned into vectors which have two phage promoters in opposite orientation. RNA can be selectively synthesized from either strand of the insert DNA with different polymerases. Homogeneously labeled single-stranded RNA can be generated with this system. Transcripts can be non-radioactively labeled with biotin or DIG-11-UTP or radioactively labeled to high specific activity with [alpha-32P] or [alpha-35S]-labeled nucleotides.Synthesis of hybridization probes T7 RNA polymerase allows highly efficient production of homogeneously labeled RNA. This labeled RNA may be used as hybridization probes in Southern northern and dot blots as well as in situ hybridizations.Suitable labelsTranscripts can be nonradioactively labeled with biotin-16-UTP or DIG-11-UTP. They may also be radioactively labeled to high specific activity with [alpha-32P]- or [alpha-35S]-labeled nucleotides.
New England Biolabs, Inc. FauI – 200 units
A restriction endonuclease that recognizes the sequence CCCGCNNNN^NN_.
Bioneer AccuPower Taq PCR PreMix, 0.2 ml thin-wall 8-tube strip with attached cap, 20 µl x 480 reactions
The AccuPower Taq PCR PreMix is a convenient lyophilized PCR master mix containing Taq DNA polymerase, dNTPs, reaction buffer, tracking dye, and patented stabilizer and is aliquoted in 8-strip PCR tubes. The premix retains its activity for over a month at room temperature and is stable for two years in -20°C freezer.